Coccidioides complement fixation

How the Test is Performed:
Adult or child:
Blood is drawn from a vein ( venipuncture ), usually from the inside of the elbow or the back of the hand. The puncture site is cleaned with antiseptic, and a tourniquet (an elastic band) or blood pressure cuff is placed around the upper arm to apply pressure and restrict blood flow through the vein. This causes veins below the tourniquet to distend (fill with blood). A needle is inserted into the vein, and the blood is collected in an air-tight vial or a syringe. During the procedure, the tourniquet is removed to restore circulation. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding .

Infant or young child:
The area is cleansed with antiseptic and punctured with a sharp needle or a lancet. The blood may be collected in a pipette (small glass tube), on a slide, onto a test strip, or into a small container. Cotton or a bandage may be applied to the puncture site if there is any continued bleeding.

Serology refers to the study of serum (the fluid portion of blood) for its antibody content. An antibody defends the body against bacteria, viruses, fungus, or other foreign bodies (antigens). Certain microbial cells stimulate the body to produce these antibodies during an active infection. Antibody production increases during the course of an infection.

In the initial stage of an illness, few antibodies may be detected. For this reason, serology tests are often repeated 10 days to 2 weeks after the initial sample. In the laboratory, the antibodies react with antigens in specific ways that can be used to confirm the identity of a specific microorganism.

There are several serology techniques that can be used depending on the suspected antibodies. Serology techniques include agglutination, precipitation, complement-fixation, fluorescent antibodies, and others.

How to Prepare:
Adults:
There is no special preparation for the test.

Infants and children:
The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:



How it Feels:
When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing .

Risks:

  • excessive bleeding
  • fainting or feeling lightheaded
  • hematoma (blood accumulating under the skin)
  • infection (a slight risk any time the skin is broken)
  • multiple punctures to locate veins



Why this Test is Performed:
This test is used to detect infection with the fungus coccidioidomycosis , which can cause lung or widespread (disseminated) infection.

Normal Values:
No coccidioides antibodies are detected.

Abnormal Results:
Abnormal results indicate that coccidioides antibodies are present. This can indicate a current or prior infection. The test may be repeated to detect a rise in titer , which helps to confirm an active infection.

Cost:
The estimated cost is $70.

Special Considerations:
Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.