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 Cytology exam of sputumHow the Test is Performed:A coughed sputum sample from the lungs is obtained in a specimen container. Other methods to obtain the specimen may include bronchoscopy and transtracheal aspiration . Normal sputum contains epithelial cells from the lining of the respiratory tract. Tumors within the respiratory tract may slough cells into the sputum. The specimen is prepared in the laboratory and examined under the microscope for the presence of abnormal cells. How to Prepare: The best specimen is obtained upon awakening in the morning. Rinse your mouth with water to avoid contamination by particles from the mouth. Cough deeply from the lungs after taking several deep breaths. One teaspoon of sputum is needed for the test. Infants and children: The physical and psychological preparation you can provide for this or any test or procedure depends on your child's age, interests, previous experience, and level of trust. For specific information regarding how you can prepare your child, see the following topics as they correspond to your child's age:
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